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Nonlinear Regression Analysis Of The Dose Response Curves Using Prism, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Symptom scores from <t>a</t> <t>VAS-questionnaire</t> addressing satiety (a) and the feeling of fullness (b) after intake of a standardized breakfast meal supplemented with a drink containing either a cod protein hydrolysate (CPH) or control (casein). Results are presented for 41 healthy subjects. Time point 0 min shows values measured right after the intake of breakfast and test material. Values are presented as mean + SD. Statistically, no differences were found between CPH and control for sensation of appetite, according to the <t>tAUC</t> of postprandial scores for satiety ( P = 0.794) and the feeling of fullness ( P = 0.966).
Total Area Under The Curve (Tauc) Analysis, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Symptom scores from <t>a</t> <t>VAS-questionnaire</t> addressing satiety (a) and the feeling of fullness (b) after intake of a standardized breakfast meal supplemented with a drink containing either a cod protein hydrolysate (CPH) or control (casein). Results are presented for 41 healthy subjects. Time point 0 min shows values measured right after the intake of breakfast and test material. Values are presented as mean + SD. Statistically, no differences were found between CPH and control for sensation of appetite, according to the <t>tAUC</t> of postprandial scores for satiety ( P = 0.794) and the feeling of fullness ( P = 0.966).
Prism Exponential Growth Curve Equation, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
Curves Generated From Raw Fluorescence Data Using A 4 Parameter Variable Slope Hill Model In Graphpad Prism 7.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GraphPad Software Inc standard curves obtained by spiking known concentrations of tfv-dp in blood using graphpad prism
Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
Standard Curves Obtained By Spiking Known Concentrations Of Tfv Dp In Blood Using Graphpad Prism, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
Anova Or T Students Test Calculations Using Graphpad Prism V.9.4.1, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
Third Order Polynomial Curve Fitting Using Graphpad Prism Version 9 Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
Concentration Response Curve Fit To The Nonlinear Regression Model Using Graphpad Prism® V6.0 Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
5 Parametric Logistic Curve Equations Graphpad Prism 10.2.2, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
Dose–Response Curves Calculated With Regression Analysis And Log Transformation Using Graphpad Prism 9.1.2, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Symptom scores from a VAS-questionnaire addressing satiety (a) and the feeling of fullness (b) after intake of a standardized breakfast meal supplemented with a drink containing either a cod protein hydrolysate (CPH) or control (casein). Results are presented for 41 healthy subjects. Time point 0 min shows values measured right after the intake of breakfast and test material. Values are presented as mean + SD. Statistically, no differences were found between CPH and control for sensation of appetite, according to the tAUC of postprandial scores for satiety ( P = 0.794) and the feeling of fullness ( P = 0.966).

Journal: Food & Nutrition Research

Article Title: Acute effect of a cod protein hydrolysate on postprandial acylated ghrelin concentration and sensations associated with appetite in healthy subjects: a double-blind crossover trial

doi: 10.29219/fnr.v63.3507

Figure Lengend Snippet: Symptom scores from a VAS-questionnaire addressing satiety (a) and the feeling of fullness (b) after intake of a standardized breakfast meal supplemented with a drink containing either a cod protein hydrolysate (CPH) or control (casein). Results are presented for 41 healthy subjects. Time point 0 min shows values measured right after the intake of breakfast and test material. Values are presented as mean + SD. Statistically, no differences were found between CPH and control for sensation of appetite, according to the tAUC of postprandial scores for satiety ( P = 0.794) and the feeling of fullness ( P = 0.966).

Article Snippet: Graphical work and total area under the curve (tAUC) analysis for acylated ghrelin concentrations and symptom VAS-scores were conducted in GraphPad.

Techniques: Control

Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.

Journal: Toxicological Sciences

Article Title: Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells

doi: 10.1093/toxsci/kfx234

Figure Lengend Snippet: Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.

Article Snippet: EC20/50 values were estimated using curves generated from raw fluorescence data using a 4-parameter variable-slope Hill model in GraphPad Prism 7.0.

Techniques: Inhibition, Activity Assay, Reporter Gene Assay, Reporter Assay, Positive Control, Intra Assay, Concentration Assay